Pickoff and spin-conversion quenchings of ortho-positronium in oxygen

The quenching processes of the thermalized ortho-positronium(o-Ps) on an oxygen molecule have been studied by the positron annihilation age-momentum correlation techinique(AMOC). The Doppler broadening spectrum of the 511 keV gamma-rays from the 2gamma annihilation of o-Ps in O_2 has been measured as a function of the o-Ps age. The rate of the quenching, consisting of the pickoff and the spin-conversion, is estimated from the positron lifetime spectrum. The ratio of the pickoff quenching rate to the spin-conversion rate is deduced from the Doppler broadening of the 511 keV gamma-rays from the annihilation of the o-Ps. The pickoff parameter ^1Z_eff, the effective number of the electrons per molecule which contribute to the pickoff quenching, for O_2 is determined to be 0.6 +- 0.4. The cross-section for the elastic spin-conversion quenching is determined to be (1.16 +- 0.01) * 10^{-19} cm^2.Comment: 4 pages with 5 eps figures, LaTeX2e(revtex4

Functional characterization and discovery of modulators of SbMATE, the agronomically important aluminium tolerance transporter from Sorghum bicolor.

About 50% of the world's arable land is strongly acidic (pH ≤ 5). The low pH solubilizes root-toxic ionic aluminium (Al3+) species from clay minerals, driving the evolution of counteractive adaptations in cultivated crops. The food crop Sorghum bicolor upregulates the membrane-embedded transporter protein SbMATE in its roots. SbMATE mediates efflux of the anionic form of the organic acid, citrate, into the soil rhizosphere, chelating Al3+ ions and thereby imparting Al-resistance based on excluding Al+3 from the growing root tip. Here, we use electrophysiological, radiolabeled, and fluorescence-based transport assays in two heterologous expression systems to establish a broad substrate recognition profile of SbMATE, showing the proton and/or sodium-driven transport of 14C-citrate anion, as well as the organic monovalent cation, ethidium, but not its divalent analog, propidium. We further complement our transport assays by measuring substrate binding to detergent-purified SbMATE protein. Finally, we use the purified membrane protein as an antigen to discover native conformation-binding and transport function-altering nanobodies using an animal-free, mRNA/cDNA display technology. Our results demonstrate the utility of using Pichia pastoris as an efficient eukaryotic host to express large quantities of functional plant transporter proteins. The nanobody discovery approach is applicable to other non-immunogenic plant proteins

Mapping the Polarization of the Radio-Loud Lyα\alpha Nebula B3 J2330+3927

Lya nebulae, or "Lya blobs", are extended (up to ~100 kpc), bright (L[Lya] > 10^43 erg/s) clouds of Lya emitting gas that tend to lie in overdense regions at z ~ 2--5. The origin of the Lya emission remains unknown, but recent theoretical work suggests that measuring the polarization might discriminate among powering mechanisms. Here we present the first narrowband, imaging polarimetry of a radio-loud Lya nebula, B3 J2330+3927 at z=3.09, with an embedded active galactic nucleus (AGN). The AGN lies near the blob's Lya emission peak and its radio lobes align roughly with the blob's major axis. With the SPOL polarimeter on the 6.5m MMT telescope, we map the total (Lya + continuum) polarization in a grid of circular apertures of radius 0.6" (4.4kpc), detecting a significant (>2sigma) polarization fraction P in nine apertures and achieving strong upper-limits (as low as 2%) elsewhere. P increases from <2% at ~5kpc from the blob center to ~17% at ~15-25kpc. The detections are distributed asymmetrically, roughly along the nebula's major axis. The polarization angles theta are mostly perpendicular to this axis. Comparing the Lya flux to that of the continuum, and conservatively assuming that the continuum is highly polarized (20-100%) and aligned with the total polarization, we place lower limits on the polarization of the Lya emission P(Lya) ranging from no significant polarization at ~5 kpc from the blob center to ~ 3--17% at 10--25kpc. Like the total polarization, the Lya polarization detections occur more often along the blob's major axis.Comment: 9 pages, 7 figures, accepted for publication in Ap

ROMA (Rank-Ordered Multifractal Analysis) for intermittent fluctuations with global crossover behavior

Rank-Ordered Multifractal Analysis (ROMA), a recently developed technique that combines the ideas of parametric rank ordering and one parameter scaling of monofractals, has the capabilities of deciphering the multifractal characteristics of intermittent fluctuations. The method allows one to understand the multifractal properties through rank-ordered scaling or non-scaling parametric variables. The idea of the ROMA technique is applied to analyze the multifractal characteristics of the auroral zone electric field fluctuations observed by SIERRA. The observed fluctuations span across contiguous multiple regimes of scales with different multifractal characteristics. We extend the ROMA technique such that it can take into account the crossover behavior -- with the possibility of collapsing probability distributions functions (PDFs) -- over these contiguous regimes.Comment: 24 pages, 18 figure

Medication adherence and visit-to-visit variability of systolic blood pressure in African Americans with chronic kidney disease in the AASK trial

Lower adherence to antihypertensive medications may increase visit-to-visit variability of blood pressure (VVV of BP), a risk factor for cardiovascular events and death. We used data from the African American Study of Kidney Disease and Hypertension (AASK) trial to examine whether lower medication adherence is associated with higher systolic VVV of BP in African Americans with hypertensive chronic kidney disease (CKD). Determinants of VVV of BP were also explored. AASK participants (n=988) were categorized by self-report or pill count as having perfect (100%), moderately high (75–99%), moderately low (50–74%) or low ( < 50%) proportion of study visits with high medication adherence over a 1-year follow-up period. We used multinomial logistic regression to examine determinants of medication adherence, and multivariable-adjusted linear regression to examine the association between medication adherence and systolic VVV of BP, defined as the coefficient of variation or the average real variability (ARV). Participants with lower self-reported adherence were generally younger and had a higher prevalence of comorbid conditions. Compared with perfect adherence, moderately high, moderately low and low adherence was associated with 0.65% (±0.31%), 0.99% (±0.31%) and 1.29% (±0.32%) higher systolic VVV of BP (defined as the coefficient of variation) in fully adjusted models. Results were qualitatively similar when using ARV or when using pill counts as the measure of adherence. Lower medication adherence is associated with higher systolic VVV of BP in African Americans with hypertensive CKD; efforts to improve medication adherence in this population may reduce systolic VVV of BP

Overcoming data scarcity of Twitter: using tweets as bootstrap with application to autism-related topic content analysis

Notwithstanding recent work which has demonstrated the potential of using Twitter messages for content-specific data mining and analysis, the depth of such analysis is inherently limited by the scarcity of data imposed by the 140 character tweet limit. In this paper we describe a novel approach for targeted knowledge exploration which uses tweet content analysis as a preliminary step. This step is used to bootstrap more sophisticated data collection from directly related but much richer content sources. In particular we demonstrate that valuable information can be collected by following URLs included in tweets. We automatically extract content from the corresponding web pages and treating each web page as a document linked to the original tweet show how a temporal topic model based on a hierarchical Dirichlet process can be used to track the evolution of a complex topic structure of a Twitter community. Using autism-related tweets we demonstrate that our method is capable of capturing a much more meaningful picture of information exchange than user-chosen hashtags.Comment: IEEE/ACM International Conference on Advances in Social Networks Analysis and Mining, 201

High pressure evolution of Fe2_{2}O3_{3} electronic structure revealed by X-ray absorption

We report the first high pressure measurement of the Fe K-edge in hematite (Fe$_2$O$_3$) by X-ray absorption spectroscopy in partial fluorescence yield geometry. The pressure-induced evolution of the electronic structure as Fe$_2$O$_3$ transforms from a high-spin insulator to a low-spin metal is reflected in the x-ray absorption pre-edge. The crystal field splitting energy was found to increase monotonically with pressure up to 48 GPa, above which a series of phase transitions occur. Atomic multiplet, cluster diagonalization, and density-functional calculations were performed to simulate the pre-edge absorption spectra, showing good qualitative agreement with the measurements. The mechanism for the pressure-induced phase transitions of Fe$_2$O$_3$ is discussed and it is shown that ligand hybridization significantly reduces the critical high-spin/low-spin pressure.Comment: 5 pages, 4 figures and 1 tabl